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tomoda

Tomo-seq data analysis

Bioconductor version: 3.23 · Package version: 1.22.0

This package provides many easy-to-use methods to analyze and visualize tomo-seq data. The tomo-seq technique is based on cryosectioning of tissue and performing RNA-seq on consecutive sections. (Reference: Kruse F, Junker JP, van Oudenaarden A, Bakkers J. Tomo-seq: A method to obtain genome-wide expression data with spatial resolution. Methods Cell Biol. 2016;135:299-307. doi:10.1016/bs.mcb.2016.01.006) The main purpose of the package is to find zones with similar transcriptional profiles and spatially expressed genes in a tomo-seq sample. Several visulization functions are available to create easy-to-modify plots.

Installation

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("tomoda")

Details

MaintainerWendao Liu <liuwd15@tsinghua.org.cn>
AuthorWendao Liu [aut, cre] (ORCID: <https://orcid.org/0000-0002-5124-9338>)
LicenseMIT + file LICENSE
URLhttps://github.com/liuwd15/tomoda
Bug Reportshttps://github.com/liuwd15/tomoda/issues
Downloads rank236
Source branchRELEASE_3_23
biocViewsClustering, GeneExpression, RNASeq, Sequencing, Software, Spatial, Transcriptomics, Visualization

Documentation

Download

Dependencies

Depends: R (>= 4.0.0)

Imports: methods, stats, grDevices, reshape2, Rtsne, umap, RColorBrewer, ggplot2, ggrepel, SummarizedExperiment

Suggests: knitr, rmarkdown, BiocStyle, testthat