tomoda
Tomo-seq data analysis
Bioconductor version: 3.23 · Package version: 1.22.0
This package provides many easy-to-use methods to analyze and visualize tomo-seq data. The tomo-seq technique is based on cryosectioning of tissue and performing RNA-seq on consecutive sections. (Reference: Kruse F, Junker JP, van Oudenaarden A, Bakkers J. Tomo-seq: A method to obtain genome-wide expression data with spatial resolution. Methods Cell Biol. 2016;135:299-307. doi:10.1016/bs.mcb.2016.01.006) The main purpose of the package is to find zones with similar transcriptional profiles and spatially expressed genes in a tomo-seq sample. Several visulization functions are available to create easy-to-modify plots.
Installation
if (!require("BiocManager", quietly = TRUE))
install.packages("BiocManager")
BiocManager::install("tomoda") Details
| Maintainer | Wendao Liu <liuwd15@tsinghua.org.cn> |
| Author | Wendao Liu [aut, cre] (ORCID: <https://orcid.org/0000-0002-5124-9338>) |
| License | MIT + file LICENSE |
| URL | https://github.com/liuwd15/tomoda |
| Bug Reports | https://github.com/liuwd15/tomoda/issues |
| Downloads rank | 236 |
| Source branch | RELEASE_3_23 |
| biocViews | Clustering, GeneExpression, RNASeq, Sequencing, Software, Spatial, Transcriptomics, Visualization |
Documentation
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Dependencies
Depends: R (>= 4.0.0)
Imports: methods, stats, grDevices, reshape2, Rtsne, umap, RColorBrewer, ggplot2, ggrepel, SummarizedExperiment