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posDemux

Positional combinatorial sequence demultiplexer

Bioconductor version: 3.23 · Package version: 1.0.0

Demultiplexing and filtering utilities intended for reads with combinatorial barcodes (i.e. PETRI-seq and SPLiT-seq). The demultiplexer algorithm uses the position of the segments to extract and compare the barcodes with the reference (whitelist). A Shiny application is provided to interactively select cutoffs for which barcode combinations to keep.

Installation

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("posDemux")

Details

MaintainerJakob Peder Pettersen <jakobpeder.pettersen@gmail.com>
AuthorJakob Peder Pettersen [aut, cre] (ORCID: <https://orcid.org/0000-0002-3485-1634>), Centre for new antibacterial strategies (CANS) [fnd]
LicenseAGPL (>= 3)
URLhttps://github.com/yaccos/posDemux, https://yaccos.github.io/posDemux/
Bug Reportshttps://github.com/yaccos/posDemux/issues
Downloads rank58
Source branchRELEASE_3_23
biocViewsRNASeq, SequenceMatching, Sequencing, Software

Documentation

Download

Dependencies

Depends: R (>= 4.5.0)

Imports: Biostrings, ggplot2, methods, assertthat, glue, magrittr, dplyr, rlang, ShortRead, readr, shiny, purrr

LinkingTo: Rcpp, Biostrings, IRanges, S4Vectors, XVector

Suggests: testthat, devtools, DNABarcodes, knitr, rmarkdown, tibble, tidyr, BiocStyle, RefManageR, sessioninfo, DBI, chunked, RSQLite, dbplyr