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methylKit

DNA methylation analysis from high-throughput bisulfite sequencing results

Bioconductor version: 3.23 · Package version: 1.38.0

methylKit is an R package for DNA methylation analysis and annotation from high-throughput bisulfite sequencing. The package is designed to deal with sequencing data from RRBS and its variants, but also target-capture methods and whole genome bisulfite sequencing. It also has functions to analyze base-pair resolution 5hmC data from experimental protocols such as oxBS-Seq and TAB-Seq. Methylation calling can be performed directly from Bismark aligned BAM files.

Installation

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("methylKit")

Details

MaintainerAltuna Akalin <aakalin@gmail.com>, Alexander Blume <alex.gos90@gmail.com>
AuthorAltuna Akalin [aut, cre], Matthias Kormaksson [aut], Sheng Li [aut], Arsene Wabo [ctb], Adrian Bierling [aut], Alexander Blume [aut], Katarzyna Wreczycka [ctb]
LicenseArtistic-2.0
URLhttps://github.com/al2na/methylKit
Bug Reportshttps://github.com/al2na/methylKit/issues
System RequirementsGNU make
Downloads rank1246
Source branchRELEASE_3_23
biocViewsDNAMethylation, MethylSeq, Sequencing, Software

Documentation

Download

Dependencies

Depends: R (>= 3.5.0), GenomicRanges (>= 1.18.1), methods

Imports: IRanges, data.table (>= 1.9.6), parallel, S4Vectors (>= 0.13.13), Seqinfo, KernSmooth, qvalue, emdbook, Rsamtools, gtools, fastseg, rtracklayer, mclust, mgcv, Rcpp, R.utils, limma, grDevices, graphics, stats, utils

LinkingTo: Rcpp, Rhtslib (>= 1.13.1)

Suggests: testthat (>= 2.1.0), knitr, rmarkdown, genomation, BiocManager

Reverse dependencies

Imports Me (3): deconvR, methInheritSim, methylInheritance