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hermes

Preprocessing, analyzing, and reporting of RNA-seq data

Bioconductor version: 3.23 · Package version: 1.16.0

Provides classes and functions for quality control, filtering, normalization and differential expression analysis of pre-processed `RNA-seq` data. Data can be imported from `SummarizedExperiment` as well as `matrix` objects and can be annotated from `BioMart`. Filtering for genes without too low expression or containing required annotations, as well as filtering for samples with sufficient correlation to other samples or total number of reads is supported. The standard normalization methods including cpm, rpkm and tpm can be used, and 'DESeq2` as well as voom differential expression analyses are available.

Installation

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("hermes")

Details

MaintainerDaniel Sabanés Bové <daniel.sabanes_bove@rconis.com>
AuthorDaniel Sabanés Bové [aut, cre], Namrata Bhatia [aut], Stefanie Bienert [aut], Benoit Falquet [aut], Haocheng Li [aut], Jeff Luong [aut], Lyndsee Midori Zhang [aut], Alex Richardson [aut], Simona Rossomanno [aut], Tim Treis [aut], Mark Yan [aut], Naomi Chang [aut], Chendi Liao [aut], Carolyn Zhang [aut], Joseph N. Paulson [aut], F. Hoffmann-La Roche AG [cph, fnd]
LicenseApache License 2.0
URLhttps://insightsengineering.github.io/hermes/
Bug Reportshttps://github.com/insightsengineering/hermes/issues
Downloads rank334
Source branchRELEASE_3_23
biocViewsDifferentialExpression, Normalization, Preprocessing, QualityControl, RNASeq, Software

Documentation

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Dependencies

Depends: ggfortify, R (>= 4.1), SummarizedExperiment (>= 1.16)

Imports: assertthat, Biobase, BiocGenerics, biomaRt, checkmate (>= 2.1), circlize, ComplexHeatmap, DESeq2, dplyr, edgeR, EnvStats, forcats (>= 1.0.0), GenomicRanges, ggplot2, ggrepel (>= 0.9), IRanges, limma, magrittr, matrixStats (>= 1.5.0), methods, MultiAssayExperiment, purrr, R6, Rdpack (>= 2.6.2), rlang, S4Vectors, stats, tidyr, utils

Suggests: BiocStyle, DelayedArray, DT, grid, httr, knitr, rmarkdown, statmod, testthat (>= 3.2.2), vdiffr (>= 1.0.8)