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demuxmix

Demultiplexing oligo-barcoded scRNA-seq data using regression mixture models

Bioconductor version: 3.23 · Package version: 1.14.0

A package for demultiplexing single-cell sequencing experiments of pooled cells labeled with barcode oligonucleotides. The package implements methods to fit regression mixture models for a probabilistic classification of cells, including multiplet detection. Demultiplexing error rates can be estimated, and methods for quality control are provided.

Installation

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("demuxmix")

Details

MaintainerHans-Ulrich Klein <hansulrich.klein@gmail.com>
AuthorHans-Ulrich Klein [aut, cre] (ORCID: <https://orcid.org/0000-0002-6382-9428>)
LicenseArtistic-2.0
URLhttps://github.com/huklein/demuxmix
Bug Reportshttps://github.com/huklein/demuxmix/issues
Downloads rank251
Source branchRELEASE_3_23
biocViewsClassification, Preprocessing, Regression, Sequencing, SingleCell, Software

Documentation

Download

Dependencies

Depends: R (>= 4.0.0)

Imports: stats, MASS, Matrix, ggplot2, gridExtra, methods

Suggests: BiocStyle, cowplot, DropletUtils, knitr, reshape2, rmarkdown, testthat (>= 3.0.0)

Reverse dependencies

Imports Me (1): demuxSNP