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HumanRetinaLRSData

Long-read RNA-seq gene count data from human retinal organoids

Bioconductor version: 3.23 · Package version: 1.0.0

Dataset package containing gene and isoform count matrices, and sample metadata for long-read direct cDNA sequencing of human retinal organoids, 2D retinal ganglion cell (RGC) cultures, and flowthrough fractions from H9 and EP1 iPSC cell lines. Data were generated using Oxford Nanopore Technology (ONT) direct cDNA sequencing and mapped to the GRCh38 reference genome (GENCODE v46 annotation). The package provides accessor functions returning SummarizedExperiment objects for gene-level counts, isoform-level counts, and a matrix of allele-specific expression (ASE) gene counts. Data files are stored in flat CSV format in an Open Science Framework (OSF) repository and cached locally via BiocFileCache.

Installation

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

BiocManager::install("HumanRetinaLRSData")

Details

MaintainerSowmya Parthiban <sparthi1@jhu.edu>
AuthorSowmya Parthiban [aut, cre]
LicenseMIT + file LICENSE
URLhttps://github.com/sparthib/HumanRetinaLrsData
Bug Reportshttps://github.com/sparthib/HumanRetinaLrsData/issues
Downloads rank20
Source branchRELEASE_3_23
biocViewsCellCulture, ExperimentData, ExpressionData, Homo_sapiens_Data, RNASeqData

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Dependencies

Depends: R (>= 4.6.0), SummarizedExperiment

Imports: osfr, BiocFileCache

Suggests: testthat, knitr, rmarkdown, ggplot2, ggrepel